Nanoparticle−Target Interactions Parallel Antibody−Protein Interactions
- 26 March 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 81 (9), 3618-3622
- https://doi.org/10.1021/ac802717c
Abstract
Magnetic particles can act as magnetic relaxation switches (MRSw’s) when they bind to target analytes, and switch between their dispersed and aggregated states resulting in changes in the spin−spin relaxation time (T2) of their surrounding water protons. Both nanoparticles (NPs, 10−100 nm) and micrometer-sized particles (MPs) have been employed as MRSw’s, to sense drugs, metabolites, oligonucleotides, proteins, bacteria, and mammalian cells. To better understand how NPs or MPs interact with targets, we employed as a molecular recognition system the reaction between the Tag peptide of the influenza virus hemagglutinin and a monoclonal antibody to that peptide (anti-Tag). To obtain targets of different size and valency, we attached the Tag peptide to BSA (Mw= 65000 Daltons, diameter = 8 nm) and to Latex spheres (diameter = 900 nm). To obtain magnetic probes of very different sizes, anti-Tag was conjugated to 40 nm NPs and 1 μm MPs. MP and NP probes reacted with Tag peptide targets in a manner similar to antibody/antigen reactions in solution, exhibiting so-called Prozone effects. MPs detected all types of targets with higher sensitivity than NPs with targets of higher valency being better detected than those of lower valency. The Tag/anti Tag recognition system can be used to synthesize combinations of molecular targets and magnetic probes, to more fully understand the aggregation reaction that occurs when probes bind targets in solution and the ensuing changes in water relaxation times that result.Keywords
This publication has 16 references indexed in Scilit:
- Chip–NMR biosensor for detection and molecular analysis of cellsNature Medicine, 2008
- Electrode Chemistry Yields a Nanoparticle-Based NMR Sensor for CalciumLangmuir, 2008
- Sensitive NMR Sensors Detect Antibodies to InfluenzaAngewandte Chemie International Edition, 2008
- Magnetic microparticle aggregation for viscosity determination by MRMagnetic Resonance in Medicine, 2008
- Multiparameter Magnetic Relaxation Switch AssaysAnalytical Chemistry, 2007
- Magnetic Relaxation Switch Detection of Human Chorionic GonadotrophinBioconjugate Chemistry, 2007
- Acceleration of the recognition rate between grafted ligands and receptors with magnetic forcesProceedings of the National Academy of Sciences, 2006
- Viral-Induced Self-Assembly of Magnetic Nanoparticles Allows the Detection of Viral Particles in Biological MediaJournal of the American Chemical Society, 2003
- Magnetic relaxation switches capable of sensing molecular interactionsNature Biotechnology, 2002
- A theory of precipitation and agglutination reactions in immunological systemsJournal of Theoretical Biology, 1974