Polyamines in Entamoeba invadens
- 1 April 1984
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 2 (2), 115-118
- https://doi.org/10.1002/cbf.290020212
Abstract
The polyamine content of Entamoeba was measured by a procedure that involved benzoylation followed by high performance liquid chromatography (h.p.l.c.). A high concentration of putrescine and significant amounts of spermidine and spermine were found in actively growing trophozoites and in the cyst forms of the organism. In contrast, trophozoites in stationary phase had greatly reduced amounts of putrescine and exhibited peaks in h.p.l.c., possibly indicative of acetylated polyamines. α‐D,L‐difluoromethylornithine (DFMO) lowered the concentration of polyamines in growing trophozoites, but did not inhibit the degree of proliferation. There is evidence for pathways of polyamine biosynthesis in Entamoeba other than through ornithine decarboxylase (ODC).Keywords
This publication has 16 references indexed in Scilit:
- Histochemical and cytochemical demonstration of ornithine decarboxylaseCell Biochemistry and Function, 1984
- Entamoeba invadens and E. histolytica: Separation and purification of precysts and cysts by centrifugation on discontinuous density gradients of percollExperimental Parasitology, 1983
- In vivo effects of α-dl-Difluoromethylornithine on the metabolism and morphology of Trypanosoma brucei bruceiMolecular and Biochemical Parasitology, 1983
- Analysis of Polyamines in Higher Plants by High Performance Liquid ChromatographyPlant Physiology, 1982
- Leishmania spp.: Effect of inhibitors on growth and on polyamine and macromolecular synthesesExperimental Parasitology, 1979
- High-pressure liquid chromatographic determination of putrescine, cadaverine, spermidine and spermineJournal of Chromatography A, 1979
- Polyamines in rapid growth and cancerBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1978
- A new medium for the axenic cultivation of Entamoeba histolytica and other EntamoebaTransactions of the Royal Society of Tropical Medicine and Hygiene, 1978
- Morphology and Life-cycle of an Amoeba Producing Amoebiasis in ReptilesParasitology, 1936