Liquid Chromatography−Tandem Mass Spectrometry of Some Anabolic Steroids
- 9 September 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (20), 4701-4710
- https://doi.org/10.1021/ac981073s
Abstract
In this paper a procedure is described for the analysis of 36 anabolic steroids, regularly found in kidney fat matrixes. After preparative HPLC is carried out, six fractions, containing the different steroids, were obtained. These fractions were then separated and on-line mass-analyzed, using APcI LC/MS. When used in tandem mass spectrometry (MRM) mode, it is possible to obtain detection limits below 1 ppb for all steroids and below 0.1 ppb for most of them. As such, it is possible to meet the regulations for detection of these anabolic steroids. To verify the influence of matrixes, real-world samples were analyzed in the same manner. This resulted in almost analog detection limits.Keywords
This publication has 14 references indexed in Scilit:
- Gas chromatography–mass spectrometric confirmation of anabolic compounds in injection sitesThe Analyst, 1994
- Veterinary drugs: disposition, biotransformation and risk evaluationThe Analyst, 1994
- Preliminary study of the metabolism of 17α-methyltestosterone in horses utilizing gas chromatography–mass spectrometric techniquesThe Analyst, 1994
- Developments in residue assay and metabolism study of growth-promoters by mass spectrometric analysisThe Analyst, 1994
- Mass spectrometry for the analysis of carcinogen-DNA adductsMass Spectrometry Reviews, 1992
- Determination of 19-nortestosterone, testosterone and trenbolone by gas chromatography—negative-ion mass spectrometry after formation of the pentafluorobenzylcarboxymethoxime—trimethylsilyl derivativesJournal of Chromatography A, 1991
- Cortisol production rates measured by liquid chromatography/mass spectrometrySteroids, 1990
- Thermospray HPLC/MS: A new mass spectrometric technique for the profiling of steroidsJournal of Steroid Biochemistry, 1987
- HPLC column switching technique for the clean-up of steroid samplesZeitschrift für Lebensmittel-Untersuchung und Forschung, 1984
- Systèmes fonciers et développement économiqueRevue économique, 1981