FILTER PAPER ELECTROPHORESIS OF HUMAN HEMOGLOBIN
Open Access
- 1 November 1953
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 32 (11), 1070-1076
- https://doi.org/10.1172/jci102829
Abstract
An adaptation of the method of Kunkel and Tiselius for the filter paper electrophoresis of human hemoglobin is descr. Methods are presented for the qualitative, semiquantitative, and quantitative analysis of human hemoglobin with this technique. The effects of time of run, voltage, buffer pH, volume, concn., dialysis, conversion to carboxyhemoglobin, size of paper, and glass plates are discussed. Data on calibration curves and recovery expts. using the semi-quantitative and quantitative techniques are presented. Examples are given of the application of this technique to the detection of normal, sickle, and C hemoglobins.Keywords
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