Lens Fiber Differentiation and Gamma Crystallins: Immunofluorescent Study of Wolffian Regeneration
- 12 March 1965
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 147 (3663), 1299-1301
- https://doi.org/10.1126/science.147.3663.1299
Abstract
From the adult lens of Triturus viridescens, a fraction of proteins was isolated which corresponds to γ-crystallins of higher vertebrates. Tests by immuno-electrophoresis indicate that the antiserum against this fraction reacts with γ-crystallins, but not with α- or β-crystallins. With this antiserum, an immunofluorescent reagent has been prepared for detection of γ-crystallins from newts. In the normal lens of the adult newt, these crystallins are detected in fiber cells and fiber material, but not in the epithelial cells. During transformation of the iris into the lens after lens removal, the staining reaction is negative in the regenerating tissue up to the time the prospective primary fiber cells begin to elongate. Subsequently, without exception those cells in fiber differentiation indicate a γ-crystallin reaction. When the secondary fiber cells are produced at the equatorial zone of the regenerating lens, they also begin to show a γ-crystallin reaction. Thus, γ-crystallins characterize fiber differentiation.Keywords
This publication has 17 references indexed in Scilit:
- An electron microscopic study of Wolffian lens regeneration in the adult newtJournal of Ultrastructure Research, 1964
- ELECTRON MICROSCOPIC STUDIES ON LENS REGENERATIONDevelopment, Growth & Differentiation, 1964
- THE INFLUENCE OF LENS ANTIBODY ON THE LENS REGENERATION IN THE LARVAL NEWTDevelopment, Growth & Differentiation, 1964
- ALKALINE PHOSPHATASE IN THE DORSAL AND THE VENTRAL HALVES OF THE IRIS DURING EARLY STAGES OF LENS-REGENERATION IN THE NEWTDevelopment, Growth & Differentiation, 1963
- APPEARANCE AND LOCALIZATION OF LENS ANTIGENS DURING THE LENS REGENERATION IN THE ADULT NEWTDevelopment, Growth & Differentiation, 1963
- Nuclear RNA synthesis in newt iris cells engaged in regenerative transformation into lens cellsDevelopmental Biology, 1963
- STUDIES ON THE LENS-REGENERATION IN THE LARVAL NEWT LACKING PIGMENT OF THE EYE1)Development, Growth & Differentiation, 1962
- Studies on γ-crystallin from calf lensExperimental Eye Research, 1961
- 32P‐UPTAKE BY DORSAL AND VENTRAL HALVES OF THE IRIS AT THE BEGINNING OF LENS REGENERATION IN THE NEWT*Development, Growth & Differentiation, 1960
- The regeneration of lenses in eyes with intact and regenerating retina in adult Triturus. v. viridescensJournal of Experimental Zoology, 1953