Fluorescence modulation of long DNA molecules adsorbed onto a microelectrode surface
- 15 December 2003
- journal article
- research article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 83 (24), 5086-5088
- https://doi.org/10.1063/1.1633333
Abstract
Using microfabricated electrodes, we demonstrate fluorescence modulation of deoxyribonucleic acid(DNA) stained with dye through the application of cyclic electric fields. The fluorescence of DNA adsorbed onto an electrode was quenched under the potentials greater than about 1 V, and was recovered by reducing the potentials to below about 0.6 V. The quenching was observed even for a single DNA molecule adsorbed on an electrode. Therefore, the fluorescence modulation of DNA by external electric fields can be expected as one of selective detection methods for adsorbed DNA onto electrodes.Keywords
This publication has 5 references indexed in Scilit:
- Fluorescence Emission Control of Long DNA Molecules Adsorbed on Microelectrode Surfaces by External VoltageJapanese Journal of Applied Physics, 2003
- DNA Sensing on a DNA Probe-Modified Electrode Using Ferrocenylnaphthalene Diimide as the Electrochemically Active LigandAnalytical Chemistry, 2000
- Electrochemical studies of the interaction of metal chelates with DNA. 4. Voltammetric and electrogenerated chemiluminescent studies of the interaction of tris(2,2'-bipyridine)osmium(II) with DNAAnalytical Chemistry, 1990
- Electrochemical investigations of the interaction of metal chelates with DNA. 3. Electrogenerated chemiluminescent investigation of the interaction of tris(1,10-phenanthroline)ruthenium(II) with DNABioconjugate Chemistry, 1990
- Electrostatic manipulation of DNA in microfabricated structuresIEEE Transactions on Industry Applications, 1990