NON′SPECIFIC AUGMENTATION WITH A HIGHLY PURI′FIED FOLLICLE STIMULATING HORMONE FRACTION (EVANS)

Abstract

An unfractionated pituitary gonadotrophic hormone prep. was not appreciably adsorbed by added Zn(0H)2 and did not manifest non-specific augmentation in the presence of such added Zn(OH)2. The hormone is quantitatively precipitated by first adding the Zn as the sulfate and then precipitating the Zn as hydroxide. Under these conditions marked non-specific augmentation is produced. A highly purified follicle stimulating hormone fraction, prepared in the laboratory of H. M. Evans, produced augmentation (150 to 550%) when administered with Zn or Cu under described conditions. This follicle stimulating hormone prep., when given intraperit. with a simultaneous subcut. inj. of the same prep, in Cu combination, gave rise to the antagonist phenomenon, viz., the preparation, by a rapid resorption rate, was able to antagonize its own influence when acting under a delayed resorption rate. In spite of all efforts at purification, the multiple hormone effects were produced by the so-called follicle stimulating hormone fraction.