The mitochondrial S13 ribosomal protein gene is silent in wheat embryos and seedlings
Open Access
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (24), 10393-10404
- https://doi.org/10.1093/nar/15.24.10393
Abstract
The sequence of a wheat mitochondrial reading frame encoding a protein homologous to the E.coli S13 small subunit ribosomal protein has been determined. The gene is located immediately downstream of a 1.4 kb recombinationally-active repeat element that contains the ATPase subunit 6 gene. The coding regions of the two genes are separated by only 153 bp, the shortest distance yet observed between protein-coding genes in plant mitochondria. However, their transcript profiles differ markedly. The ATPase 6 gene displays a single, prominent mRNA of approximately 1.4 kb, whereas the S13 gene shows no stable transcript as judged by Northern blot analysis of wheat mitochondrial RNA isolated from different developmental stages. A short segment of the 26S rRNA gene is located downstream of the S13 gene and its presence illustrates the frequent DNA duplication/rearrangements found in wheat mitochondria.Keywords
This publication has 23 references indexed in Scilit:
- Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the texas male-sterile cytoplasmCell, 1986
- Nucleotide Sequence of ATPase Subunit 6 Gene of Maize MitochondriaPlant Physiology, 1985
- The Unusual varl Gene of Yeast Mitochondrial DNAScience, 1985
- TRANSCRIPTION OF THE MAMMALIAN MITOCHONDRIAL GENOMEAnnual Review of Biochemistry, 1984
- Sequence analysis of the maize mitochondrial 26 S rRNA gene and flanking regionsPlasmid, 1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- The making of strand-specific M13 probesGene, 1982
- The zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codonsCell, 1981
- Mitochondrial ribosome assembly in Neurospora. Two-dimensional gel electrophoretic analysis of mitochondrial ribosomal proteins.The Journal of cell biology, 1979
- Primary structure of protein S13 from the small ribosomal subunit of Escherichia coliFEBS Letters, 1976