A Host Range Mutant of Human Adenovirus Type 5 Defective for Growth in Hamster Cells

Abstract

A host-range mutant of adenovirus type 5, which grows in human cells but not in hamster cells, was isolated. This mutant is complemented in mixed infection in hamster cells at 38.5.degree. C by temperature-sensitive mutants of type 5 belonging to 17 complementation groups, and may constitute a new group. In mixed infection of human cells at 32.degree. C with the host-range and temperature-sensitive mutants, recombination takes place and by a series of 2 factor crosses the host-range mutation was approximately located on the adenovirus genetic map.