Syngeneic t cell transfer of diabetes into nod newborn mice: in situ studies of the autoimmune steps leading to insulin‐producing cell destruction

Abstract

To overcome the limitations of in situ studies during the chronic spontaneous autoimmune process leading to insulin cell destruction and diabetes in non-obese diabetic (NOD) mice, we have developed a model of acute transfer of diabetes into healthy syngeneic newborns. The injection of 20 × 10⁶ T cells from adult diabetic mice produced synchronous insulitis within 3 weeks and diabetes within 4–5 weeks in young recipients, at a time when non-injected control mice do not even exhibit histological changes in their pancreases. Sequential studies of pancreases from T cell-transferred mice showed that lymphoid infiltration was preceded by a strong tissue expression of Ia antigen which was restricted to the vessel-associated cells limiting the islet of Langerhans, and which might play a role in the recruitment of circulating T cells inside the islets. Acute destruction of most of the insulin-producing cells, leading to diabetes, could take place within a few days after insulitis had begun. A majority of the inflammatory cells were T lymphocytes, ∼30% of which expressed interleukin 2 receptors. L3T4⁺ T cells largely predominated at the early phase of islet invasion whereas the proportion of Ly-2⁺ T cells substantially increased later when β cell destruction occurred. In contrast, only a minority of B cells and macrophages participated to the inflammatory process. These data are in keeping with previous demonstrations that both T cell subsets contribute to the autoimmune disease. Furthermore, they suggest that β cell injury is mediated through a cytotoxic process, which requires the sequential involvement of L3T4⁺ (helper) and Ly-2⁺ (cytotoxic) T cells.