THE ROLE OF IRON IN THE BIOLOGY OF BRUCELLA SUIS I
- 1 July 1953
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 66 (1), 82-91
- https://doi.org/10.1128/jb.66.1.82-91.1953
Abstract
Methods were devised for freeing culture media of traces of Fe by chelation with 8-hydroxyquinoline (oxine), followed by extraction with chloroform. Brucella suis requires at least 0.030 parts of iron per million parts of medium for max. growth. Fe-deficient B. suis fails to dissociate to the degree observed under Fe-normal conditions. Alanine accumulation is reduced markedly during Fe deficiency. Animal inoculation studies with Fe-deficient, almost catalase-free, B. suis reveal a tendency to greater invasiveness, more rapid multiplication, a more slowly developing inflammatory response of the mononuclear type, and degradation of the granulocytes which infiltrate the site of infection.Keywords
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