Respiratory syncytial virus proteins: identification by immunoprecipitation
- 1 April 1981
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 38 (1), 278-285
- https://doi.org/10.1128/jvi.38.1.278-285.1981
Abstract
Respiratory syncytial virus was pulse-labeled with [35S]methionine and [35S]cysteine and cell lysates were analyzed by polyacrylamide gel electrophoresis. Five 35S-labeled viral proteins ranging in MW from 21,000-73,000 (VP73, VP44, VP35, VP28 and VP21) were easily discernable above background cellular proteins. Treatment of the infected cells with 0.15 M NaCl before labeling suppressed host cell protein synthesis and allowed clearer visualization of the 5 viral proteins by polyacrylamide gel electrophoresis. Three glycoproteins (VGP 92, VGP 50 and VGP 17) were also identified after labeling with [3H]glucosamine. Five of these polypeptides (VP51, VP44, VP35, VP28 and VGP92) were antigenically active because they could be immunoprecipitated with anti-respiratory syncytial virus antibody produced in New Zealand white rabbits, cotton rats and humans before analysis by polyacrylamide gel electrophoresis. [Human epidermoid carcinoma HEp-2 and fetal fibroblast HFF and rhesus monkey embryo kidney MA104 cells were used in these studies.].This publication has 19 references indexed in Scilit:
- Respiratory syncytial virus polypeptides: Their location in the virionVirology, 1979
- The polypeptides of human respiratory syncytial virus: Products of cell-free protein synthesis and post-translational modificationsVirology, 1979
- Evidence for a ninth influenza viral polypeptideVirology, 1978
- A comparison of the polypeptides of human and bovine respiratory syncytial viruses and murine pneumonia virusVirology, 1977
- Respiratory syncytial virus proteinsVirology, 1976
- Separation and concentration of respiratory syncytial virus (RSV) antigensJournal of Immunological Methods, 1974
- Genetic studies of respiratory syneytial virus temperature-sensitive mutantsArchiv für die gesamte Virusforschung, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Kinetics of the respiratory syncytial virus growth cycle in Hela cellsArchiv für die gesamte Virusforschung, 1969
- The ultrastructure of respiratory syncytial (RS) virusArchiv für die gesamte Virusforschung, 1963