RAPID METHOD FOR DETERMINATION OF ALDOSTERONE IN HUMAN URINE BY GLASS PAPER CHROMATOGRAPHY*

Abstract

A method for the determination of aldosterone in urine is described, which utilizes rapid chromatography on glass fiber paper. After acid hydrolysis, chloroform extracts of the urine are chromatographed on silica-gel columns, run on 2 glass paper chromatographic systems, acetylated, and again separated by 2 additional runs in separate glass chromatographic systems. Final determination of aldosterone is performed by measurement of alkaline fluorescence. The specificity and precision of the method, the recovery of steroid by this technic, and the range of values in normal man are presented. The advantages of glass paper chromatography are pointed out, not the least important of which is that each chromatographic step requires only fifteen minutes.