A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.
Open Access
- 1 February 1992
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 1 (3), 160-163
- https://doi.org/10.1101/gr.1.3.160
Abstract
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.Keywords
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