PRIMARY STRUCTURE OF ALANYL-TRANSFER RNA-SYNTHETASE AND THE REGULATION OF ITS MESSENGER-RNA LEVELS IN BOMBYX-MORI
- 5 December 1990
- journal article
- research article
- Vol. 265 (34), 20898-20906
Abstract
CDNA clones encoding Bombyx mori alanyl-tRNA synthetase were isolated from a library in .lambda.gt11 using antibody, synthetic oligonucleotides, and a characterized cDNA as probes. Analysis of the sequence revealed significant homology between the B. mori and Escherichia coli alanyl-tRNA synthetases, particularly in their amino-terminal domains. Northern blot analysis indicated that the mRNA for alanyl-tRNA synthetase mRNA isolated from posterior silk gland, middle silk gland, and ovarian tissue. Steady-state levels of alanyl-tRNA synthetase mRNA in the posterior silk gland increased in the first 48 h of the fifth larval instar, decreasing gradually thereafter. In the middle silk gland, alanyl-tRNA synthetase mRNA peaked at 72 h of the fifth larval instar, declining to undetectable levels by 120 h. Genomic Southern blot analysis using a nick-translated cDNA probe revealed hybridization to single fragments when B. mori genomic DNA was digested with various restriction endonucleases.This publication has 34 references indexed in Scilit:
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