Association of viable and inactivated Salmonella typhimurium 395 MS and MR 10 with HeLa cells

Abstract

The mouse-virulent S. typhimurium 395 MS, containing a complete lipopolysaccharide (LPS) structure with S-specific repeating units, and the nonvirulent, LPS-defective mutant 395 MR 10 (chemotype Rd) derived from it were studied for their tendency to interact with HeLa cells. In the definition of interaction no distinction was made between intracellular and cell membrane-attached bacteria. R10 bacteria had a greater tendency to interact than MS bacteria. This difference was seen as early as 1 h after the start of incubation, but it became more pronounced beyond 3 h. Heat-killed and UV-killed R10 bacteria interacted with HeLa cells less than living ones. Killed MS bacteria interacted to an extent similar to that of living ones. These results are discussed in relation to the susceptibility of the bacteria to phagocytosis by professional phagocytic cells and to the physicochemical properties [hydrophilicity and surface charge] of the bacteria as measured by their distribution in a 2-polymer, aqueous-phase system.