Reaction of Endotoxin and Surfactants I. Physical and Biological Properties of Endotoxin Treated with Sodium Deoxycholate
- 1 November 1966
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 92 (5), 1493-+
- https://doi.org/10.1128/jb.92.5.1493-1509.1966
Abstract
Endotoxtas from 3 species of gram-negative bacteria were shown to be dissociated by the bile salt sodium deoxycholate (NaD) into nontoxic subunits with molecular weights of about 20,000. When the bile salt was removed by dialysis, the subunits reaggregated in an orderly manner to form a relatively uniform population of biologically active endotoxin particles with average molecular weights of 500,000 to 1,000,000. If a small amount of human plasma was added to the dissociated endotoxin before removal of the NaD, reassociation apparently did not occur and the preparation remained nonpyrogenic. However, the plasma protein could subsequently be removed from the endotoxin subunits, and reaggregation to the toxic form would then occur. The studies on the physical nature of endotoxin performed with biophysical solution techniques were supplemented and confirmed by direct examination of the endotoxin polymers by electron microscopy. The results of these studies were consonant with the theory that the biologically active endotoxic elements are composed of micellar aggregates of linear lipopolysaccharide subunits.This publication has 30 references indexed in Scilit:
- PHYSICAL ASPECTS OF REVERSIBLE INACTIVATION OF ENDOTOXIN*Annals of the New York Academy of Sciences, 1966
- Relationship between Particle Size and Biological Activity of E. coli Boivin Endotoxin*JCI Insight, 1965
- Restoration of Endotoxin Activity following Alteration by PlasmaNature, 1964
- Dissociation and reconstitution of an endotoxinBiochemical and Biophysical Research Communications, 1963
- Endotoxoid PreparationsNature, 1963
- BIOLOGICAL PROPERTIES OF PARENT ENDOTOXINS AND LIPOID FRACTIONS, WITH A KINETIC STUDY OF ACID-HYDROLYZED ENDOTOXINThe Journal of Experimental Medicine, 1961
- PREPARATION AND HOST-REACTIVE PROPERTIES OF ENDOTOXIN WITH LOW CONTENT OF NITROGEN AND LIPIDThe Journal of Experimental Medicine, 1961
- PREPARATION AND BIOLOGICAL PROPERTIES OF A CHEMICALLY MODIFIED ESCHERICHIA COLI ENDOTOXIN OF HIGH IMMUNOGENIC POTENCY AND LOW TOXICITYJCI Insight, 1961
- OBSERVATIONS ON THE PYROGENIC RESPONSE AND ITS APPLICATION TO THE BIOASSAY OF ENDOTOXINJCI Insight, 1961
- Viscosity and the Shape of Protein MoleculesScience, 1940