Is the ClC‐2 chloride channel involved in the Cl− secretory mechanism of gastric parietal cells?
Open Access
- 11 September 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 575 (1-3), 105-108
- https://doi.org/10.1016/j.febslet.2004.08.044
Abstract
It has been controversial whether the ClC‐2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC‐2 is the apical Cl− channel associated with gastric acid secretion. Two anti‐ClC‐2 antibodies used in this study reacted with cloned ClC‐2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC‐2 mRNA was observed, but the presence of ClC‐2 protein was not clear. Furthermore, no expression of ClC‐2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC‐2 protein in the parietal cells which showed abundant expression of H+,K+‐ATPase. These results indicate that ClC‐2 may not be a Cl−‐transporting protein for gastric acid secretion in parietal cells.Keywords
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