The origin, kinetics, and characteristics of the kupffer cells in the normal steady state
Open Access
- 1 July 1978
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 148 (1), 1-17
- https://doi.org/10.1084/jem.148.1.1
Abstract
Enzymatic digestion with pronase and DNAase was used to isolate Kupffer cells from mouse liver. The characteristics of these cells were found to be similar to those of peritoneal macrophages, except that in the initial suspension the percentage of Kupffer cells with Fc receptors was low, C receptors were absent and the ingestion of opsenized bacteria was very poor, because of the effect of pronase on the cell membrane. After 24 h incubation in vitro all these characteristics return.Keywords
This publication has 38 references indexed in Scilit:
- Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study.The Journal of cell biology, 1977
- Phagocytosis in rat Kupffer cells in vitroExperimental Cell Research, 1976
- Disparity in origin of mononuclear phagocyte populations.1976
- Distribution of lysosomal enzymes in different types of rat liver cellsExperimental Cell Research, 1976
- A serum facted by newborn calf serum.1976
- Histological Sexing of a Reticulum Cell Sarcoma Arising after Liver TransplantationGastroenterology, 1976
- Proliferation of mononuclear phagocytes (Kupffer cells) and endothelial cells in regenerating rat liver. A light and electron microscopic cytochemical study.1975
- Studies on hepatic uptake of antigen. III. Studies of liver macrophage function in normal rats and following carbon tetrachloride administration.1975
- Mass isolation and culture of rat kupffer cells.The Journal of Experimental Medicine, 1975
- THE FINE STRUCTURAL LOCALIZATION OF ENDOGENOUS AND EXOGENOUS PEROXIDASE ACTIVITY IN KUPFFER CELLS OF RAT LIVERThe Journal of cell biology, 1970