Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies
- 15 September 2011
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 193 (18), 4984-4987
- https://doi.org/10.1128/jb.00315-11
Abstract
Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.Keywords
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