Ultrafast Cleavage and Deprotection of Oligonucleotides Synthesis and Use of CAcDerivatives
- 1 July 1997
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 16 (7-9), 1589-1598
- https://doi.org/10.1080/07328319708006236
Abstract
We have investigated the use of alkylamines as fast cleavage and deprotection reagents for the solid phase synthesis of oligonucleotides and found methylamine/ammonium hydroxide (or methylamine) as an efficient reagent. The transamination side product formed with the commonly used dCbz has been eliminated by the use of dCAc phosphoramidite. This system has successfully been used in the synthesis of oligonucleotides and oligonucleoside phosphorothioates. DMT dCAc hydrogen phosphonate and DMT ribo CAc-2′-O Me phosphoramidite also have been prepared and used in the synthesis of oligonucleotides.Keywords
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