Micro-determination of cyclic AMP levels in human epidermis, dermis and hair follicles
- 1 March 1975
- journal article
- Published by Oxford University Press (OUP) in British Journal of Dermatology
- Vol. 92 (3), 241-248
- https://doi.org/10.1111/j.1365-2133.1975.tb03072.x
Abstract
In order to study the biological and possible pathological roles of cyclic adenosine 3′,5′-monophsphate (cyclic AMP) in the skin, it is mandatory to measure cyclic AMP in 50–100 μg of microdissected epidermis, dermis or appendages. In the present study, we ofter a method of extracting cyclic AMP from less than 100 μg of tissue, removing contaminating nucleotides and scaling down Gilman's method to fit the analysis of small amounts of tissue. Cyclic AMP levels in the dermis, epidermis, and hair follicles (bulbs) were approximately 1, 2 and 3.5 pmols/μg dry weight tissue respectively. This procedure is applicable to the measurement of cyclic AMP levels in limited foci of healthy or diseased skin.Keywords
This publication has 12 references indexed in Scilit:
- Cyclic adenosine monophosphate, adenyl cyclase and cyclic nucleotide phosphodiesterase in psoriatic epidermis, with a comment on urinary cAMPActa Dermato-Venereologica, 1974
- Increased cyclic GMP and decreased cyclic AMP levels in the hyperplastic, abnormally differentiated epidermis of psoriasisLife Sciences, 1973
- ATP and ADP levels and epidermal replacement rate in the normal human skin and in some papulosquamous diseases of the skinActa Dermato-Venereologica, 1973
- Decreased cyclic AMP in the epidermis of lesions of psoriasisArchives of Dermatology, 1972
- Nature and Regulation of Energy Metabolism in the EpidermisJournal of Investigative Dermatology, 1971
- Effects of phenothiazine tranquilizers on the cyclic 3′,5′-adenosine monophosphate system of rat brainNeuropharmacology, 1971
- A Protein Binding Assay for Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1970
- On the assay of adenyl cyclaseAnalytical Biochemistry, 1970
- Measurement of Adenosine 3’, 5’-Monophosphate (Cyclic AMP) in BrainPharmacology, 1970
- THE QUANTITATIVE HISTOCHEMISTRY OF THE BRAINJournal of Histochemistry & Cytochemistry, 1953