ESTIMATION OF GOLGI MEMBRANE FLOW-RATES IN OVARY GLANDS OF APTENIA-CORDIFOLIA USING CYTOCHALASIN-B
- 1 January 1983
- journal article
- research article
- Vol. 29 (2), 262-267
Abstract
Ovary gland cells of A. cordifolia were exposed to 100 .mu.g/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the plasma membrane. The ultrastructural features of the Golgi apparatus in control and cyt B-treated cells was analyzed using stereological methods to estimate the mean area of vesicular membrane produced by a single dictyosome during a 30 min period of effective cyt B action. Assuming that the rate of vesicle congestion can be equated with the rate of vesicle production, the 236 dictyosomes present in the non-growing ovary gland cells form 7517 vesicles in total, or .apprx. 32 vesicles each within a period of 30 min. This corresponds to a membrane turnover rate of 70.4 .mu.m2/min (this equals .apprx. 10% of the total plasma membrane area per min), since the mean vesicle surface area was 0.281 .mu.m2. The turnover time of a single Golgi cisterna was 7.34 min and the average vesicle life time was 8.86 min. The way by which the relatively high amount of vesicular membrane material incorporated into the plasmalemma is recycled into the endomembrane system was discussed. Since a bulk membrane retrieval in the form of vesicles, as well as a bulk vesicle migration from the ER [endoplasmic reticulum] to the dictyosomes was not present, a transfer of membrane subunits is possibly involved in the maintenance of membrane equilibrium in the Golgi apparatus.This publication has 15 references indexed in Scilit:
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