A RAPID METHOD FOR DETERMINING BLOOD VOLUMES BY THE USE OF P32-LABELLED RED CELLS

Abstract

6-8 ml. of a subject''s own blood is incubated for 30 min. with 100[um] curies P32. The cells are then washed, resuspended in sterile buffer and a measured aliquot injd. After 10-20 min. a blood sample is withdrawn and its radioactivity measured on a Geiger counter above a cup filled to a depth of over 7 mm. with the sample. This is compared with the measurement of an 18% glucose soln. dilution of the original suspension, giving the blood volume. The technic reduces errors due to plasma p32 variations, erythrocyte P32 loss, and sample beta-ray self-absorption, furnishing a method suitable for routine detns.