Predominance of Distinct Viral Genotypes in Brain and Lymph Node Compartments of HIV-1-Infected Individuals
- 13 March 2009
- journal article
- research article
- Published by Mary Ann Liebert Inc in Viral Immunology
- Vol. 4 (2), 123-131
- https://doi.org/10.1089/vim.1991.4.123
Abstract
A modified polymerase chain reaction protocol was used to amplify the entire envelope-coding region of HIV-1 directly from brain and lymph node tissue obtained at autopsy from three HIV-1-infected individuals. Molecular analysis of amplified DNA by digestion with 18 restriction endonucleases, singly and in combination, revealed different HIV-1 genotypes in the brain and lymph node compartments in each of the three individuals. This anatomic compartmentalization of HIV-1 populations may reflect different viral genomic sequences that determine tropism or differences in host immune selection pressures in the brain and lymphoid compartments that drive the emergence of distinct viral populations.Keywords
This publication has 51 references indexed in Scilit:
- Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp!20 geneNature, 1991
- HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domainNature, 1990
- Specific Tropism of HIV-1 for Microglial Cells in Primary Human Brain CulturesScience, 1990
- Molecular Cloning and Characterization of a German HIV-1 IsolateAIDS Research and Human Retroviruses, 1990
- Infection of Brain Cells by Diverse Human Immunodeficiency Virus Isolates: Role of CD4 as ReceptorJournal of General Virology, 1989
- Single amino-acid changes in HIV envelope affect viral tropism and receptor bindingNature, 1989
- Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes.The Journal of Experimental Medicine, 1988
- Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptorCell, 1987
- Susceptibility of human glial cells to infection with human immunodeficiency virus (HIV)FEBS Letters, 1987
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985