Detection of bovine αs1‐casein genomic variants using the allele‐specific polymerase chain reaction
- 1 October 1992
- journal article
- Published by Wiley in Animal Genetics
- Vol. 23 (5), 425-429
- https://doi.org/10.1111/j.1365-2052.1992.tb02161.x
Abstract
A method was developed to distinguish between genotypic variants B and C of bovine alpha s1-casein, using the allele-specific polymerase chain reaction (ASPCR). The alpha s1-casein genotype determined for 17 Jersey cows by the ASPCR method was confirmed by typing the alpha s1-casein milk proteins on isoelectric focusing gels. Using the ASPCR method described, rapid analysis of the alpha s1-casein genotype of bulls is now possible. In addition, kappa-casein genotypes can be determined from the same PCR reaction.Keywords
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