Muscle contraction-blood flow interactions during upright knee extension exercise in humans

Abstract

To test for evidence of a muscle pump effect during steady-state upright submaximal knee extension exercise, seven male subjects performed seven discontinuous, incremental exercise stages (3 min/stage) at 40 contractions/min, at work rates ranging to 60–75% peak aerobic work rate. Cardiac cycle-averaged muscle blood flow (MBF) responses and contraction-averaged blood flow responses were calculated from continuous Doppler sonography of the femoral artery. Net contribution of the muscle pump was estimated by the difference between mean exercise blood flow (MBF_M) and early recovery blood flow (MBF_R). MBF_M rose in proportion with increases in power output with no significant difference between the two methods of calculating MBF. For stages 1 and 5, MBF_M was greater than MBF_R; for all others, MBF_M was similar to MBF_R. For the lighter work rates ( stages 1–4), there was no significant difference between exercise and early recovery mean arterial pressure (MAP). During stages 5–7, MAP was significantly higher during exercise and fell significantly early in recovery. From these results we conclude that 1) at the lightest work rate, the muscle pump had a net positive effect on MBF_M, 2) during steady-state moderate exercise ( stages 2–4) the net effect of rhythmic muscle contraction was neutral (i.e., the impedance due to muscle contraction was exactly offset by the potential enhancement during relaxation), and 3) at the three higher work rates tested ( stages 5–7), any enhancement to flow during relaxation was insufficient to fully compensate for the contraction-induced impedance to muscle perfusion. This necessitated a higher MAP to achieve the MBF_M.