RNA target specificity of the STAR/GSG domain post-transcriptional regulatory protein GLD-1
- 29 December 2003
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 11 (1), 20-28
- https://doi.org/10.1038/nsmb706
Abstract
The post-transcriptional regulation of gene expression underlies several critical developmental phenomena. In metazoa, gene products that are expressed, silenced and packaged during oogenesis govern early developmental processes prior to nascent transcription activation. Furthermore, tissue-specific alternative splicing of several transcription factors controls pattern formation and organ development. A highly conserved family of proteins containing a STAR/GSG RNA-binding domain is essential to both processes. Here, we identify the consensus STAR-binding element (SBE) required for specific mRNA recognition by GLD-1, a key regulator of Caenorhabditis elegans germline development. We have identified and verified new GLD-1 repression targets containing this sequence. The results suggest additional functions of GLD-1 in X-chromosome silencing and early embryogenesis. The SBE is present in Quaking and How mRNA targets, suggesting that STAR protein specificity is highly conserved. Similarities between the SBE and the branch-site signal indicate a possible competition mechanism for STAR/GSG regulation of splicing variants.Keywords
This publication has 44 references indexed in Scilit:
- A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegansNature, 2002
- CDC‐25.1 regulates germline proliferation in Caenorhabditis elegansGenesis, 2002
- Translational Regulation and RNA Localization in Drosophila Oocytes and EmbryosAnnual Review of Genetics, 2001
- Structural Basis for Recognition of the Intron Branch Site RNA by Splicing Factor 1Science, 2001
- Central domain assembly: thermodynamics and kinetics of S6 and S18 binding to an S15-RNA complexJournal of Molecular Biology, 2001
- Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell developmentGenes & Development, 2001
- Recognition of RNA Branch Point Sequences by the KH Domain of Splicing Factor 1 (Mammalian Branch Point Binding Protein) in a Splicing Factor ComplexMolecular and Cellular Biology, 2001
- It ain't over till it's ova: germline sex determination in C. elegansBioEssays, 2001
- Interaction of theBacillus stearothermophilusRibosomal Protein S15 with 16 S rRNA: I. Defining the Minimal RNA SiteJournal of Molecular Biology, 1996
- Translational regulation in developmentCell, 1995