A STUDY OF GLYCOLYSIS

Abstract

A study of the rate of glycolysis in bloods from 80 patients with different diseases is presented. There is no correlation between age or sex and rate. The rate is markedly influenced by temp. Glycolytic studies should be performed at 37[degree] C.,[long dash]the optimum for the disappearance of sugar from blood in vitro. Citrates and oxalates, when used as anticoagulants, retard glycolysis in drawn blood in proportion to the concentration. Fluorides arrest glycolysis. Defibrination or heparinization does not interfere with the rate of glycolysis, and blood so treated should be used for glycolytic studies. The rate bears no relation to the initial glucose content of the blood. Glycolysis in vitro is not affected by insulin. Both erythrocytes and leucocytes are responsible for the glycolytic power of blood, and their number, type, age, and physiological integrity are important.