A new restriction endonudease fromSpirulina platensis
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (5), 1985-1989
- https://doi.org/10.1093/nar/14.5.1985
Abstract
Three restriction endonucleases, SplI, SplII and SplIII have been purified partially from Spirulina platensis subspecies sjampse and named. SplI cleaves bacteriophage lambda DNA at one site, phi X 174 RF DNA at two sites, but does not cleave pBR322 DNA. This enzyme recognizes the sequence 5‘CGTACG3’ and 3‘GCATCG5’ cuts the site indicated by the arrows. Spill is an isoschizomer of TthlllI and SplIII is an isoschizomer of HaeIII.Keywords
This publication has 5 references indexed in Scilit:
- Restriction and modification enzymes and their recognition sequencesNucleic Acids Research, 1985
- A site specific endonuclease from Thermus thermophilus 111, Tth1111Nucleic Acids Research, 1980
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology, 1980
- An improved method for the separation and quantitation of the modified nudeosides of transfer RNANucleic Acids Research, 1976
- A second specific endonuclease from Haemophilus aegyptiusJournal of Molecular Biology, 1975