Effect of colchicine on intestinal disaccharidases: correlation with biochemical aspects of cellular renewal
Open Access
- 1 March 1970
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 49 (3), 530-536
- https://doi.org/10.1172/jci106263
Abstract
There was a significant depression of the activities of intestinal lactase, invertase, and alkaline phosphatase in rats given drinking water containing 2.5 mg of colchicine per 100 ml. Activities of intestinal maltase, aspartate transcarbamylase, and dihydroorotase were not affected by the drug. Injection of colchicine (1 mg/kg) caused depression of intestinal invertase activity within 8 hr. Investigation of the effect of colchicine on the disaccharides in vitro demonstrated that invertase and maltase were not affected by concentrations up to 125 mg/100 ml. Intestinal lactase was inhibited by concentrations exceeding 5 mg/100 ml. Calculation of the concentration of colchicine present in the intestine, after a single injection, indicated that the in vivo effect of colchicine was not due to simple enzyme inhibition. Histological examination showed an increase in crypt cells but no decrease in the length of the villi. Cellular migration along the villi, as well as activity of uridine kinase in intestinal mucosa, was increased in colchicine-treated rats. It was concluded that colchicine did not depress intestinal invertase, lactase, and alkaline phosphatase by decreasing cellular renewal, but rather it exerted its effect directly on the differentiated cells of the villus.Keywords
This publication has 26 references indexed in Scilit:
- QUANTITATIVE DETERMINATION OF ENZYMES IN DIFFERENT PARTS OF THE VILLI AND CRYPTS OF RAT SMALL INTESTINE COMPARISON OF ALKALINE PHOSPHATASE, DISACCHARIDASES AND DIPEPTIDASESJournal of Histochemistry & Cytochemistry, 1967
- Lactose Intolerance in AsiansNature, 1967
- Effect of Diet upon Intestinal Disaccharidases and Disaccharide Absorption*JCI Insight, 1967
- Effect of colchicine on intestinal function in the rat.Gut, 1966
- Malignant Gout with Tophaceous Small Intestine and Megaloblastic AnaemiaAnnals Of The Rheumatic Diseases, 1965
- Isomaltose intolerance causing decreased ability to utilize dietary starchThe Journal of Pediatrics, 1963
- Studies of small intestine during development I. Distribution and activity of ß-galactosidaseBiochimica et Biophysica Acta, 1962
- The digestive function of the epithelium of the small intestineBiochimica et Biophysica Acta, 1961
- A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphataseBiochimica et Biophysica Acta, 1960
- DEFECTIVE LACTOSE ABSORPTION CAUSING MALNUTRITION IN INFANCYThe Lancet, 1959