Characterization of soluble forms of NCAM
Open Access
- 10 December 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 225 (1-2), 33-36
- https://doi.org/10.1016/0014-5793(87)81126-2
Abstract
Neural cell adhesion molecule (NCAM) has been described as a family of membrane glycoproteins. However, soluble NCAM immunoreactivity has long been recognized. We here show that soluble NCAM is composed of two quantitatively major polypeptides of M r 180 000 and 115 000 and two minor components of M r 160 000 and 145 000. Soluble NCAM was immunochemically identical to membrane NCAM, was polysialylated and carried the HNK‐1 epitope. It only constituted 0.8% of total NCAM in newborn rat brain. Soluble NCAM appeared in neuronal cell culture medium 15–30 min after the start of synthesis preceding accumulation of membrane‐associated NCAM on the cell surface. This indicates that soluble NCAM contains a secreted component.Keywords
This publication has 15 references indexed in Scilit:
- Release of the 120 kDa component of the mouse neural cell adhesion molecule N-CAM from cell surfaces by phosphatidylinositol-specific phospholipase CNeuroscience Letters, 1986
- Biosynthesis of the neural cell adhesion molecule: characterization of polypeptide C.The Journal of cell biology, 1985
- Biosynthesis of the D2-cell adhesion molecule: post-translational modifications, intracellular transport, and developmental changes.The Journal of cell biology, 1984
- Release of the NILE and Other Glycoproteins from Cultured PC 12 Rat Pheochromocytoma Cells and Sympathetic NeuronsJournal of Neurochemistry, 1984
- Studies on the transmembrane disposition of the neural cell adhesion molecule N‐CAMEuropean Journal of Biochemistry, 1984
- Biosynthesis of the D2 cell adhesion molecule: pulse-chase studies in cultured fetal rat neuronal cells.The Journal of cell biology, 1984
- L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellumNature, 1983
- Quantification of the D2‐Glycoprotein in Amniotic Fluid and Serum from Pregnancies with Fetal Neural Tube DefectsJournal of Neurochemistry, 1983
- Purification of the Synaptic Membrane Glycoprotein D2 from Rat BrainScandinavian Journal of Immunology, 1982
- Why a blood-brain barrier?Trends in Neurosciences, 1979