Further investigations on the metabolism of 2:3-dimercaptopropanol

Abstract

The thiols present in rabbit urine after injn. of 2:3-dimercap-topropanol (BAL) have been estimated by I titration and by reaction with Co nitrate. Extraction by means of precipitation with thallous sulphate has yielded a purified prepn. of a thiol present in post-injn. urine. Examination of the optical densities at different wave-lengths of the Co color given by the purified urinary thiol together with measurements of its absorption spectrum both in the visible and u.-v. regions of the spectrum suggest that this thiol is closely related to BAL but not identical with it. The urinary thiol is capable of protecting brain pyruvate oxidase prepns. from inhibition by lewisite at concns. at which no monothiol has yet been found to be effective.