Evidence for a second phosphorylation site on eIF‐2α from rabbit reticulocytes
- 4 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 215 (1), 16-20
- https://doi.org/10.1016/0014-5793(87)80105-9
Abstract
Ser 51 in the NH2-terminal sequence of the alpha-subunit of eukaryotic peptide initiation factor 2 (eIF-2) has been identified as a second phosphorylation site for the heme-controlled eIF-2 alpha kinase from rabbit reticulocytes. Increased phosphorylation of this serine relative to the previously described phosphorylation site (Ser 48) is observed when the kinase reaction is carried out in the presence of the alpha-subunit of spectrin. A synthetic peptide corresponding to eIF-2 alpha (41-54) is phosphorylated only in Ser 51 by the eIF-2 alpha kinase.Keywords
This publication has 8 references indexed in Scilit:
- Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA.Journal of Biological Chemistry, 1987
- The NH2-terminal sequence of the alpha and gamma subunits of eukaryotic initiation factor 2 and the phosphorylation site for the heme-regulated eIF-2 alpha kinase.Journal of Biological Chemistry, 1986
- A calmodulin and α-subunit binding domain in human erythrocyte spectrinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986
- The 90-kDa component of reticulocyte heme-regulated eIF-2 alpha (initiation factor 2 alpha-subunit) kinase is derived from the beta subunit of spectrin.Proceedings of the National Academy of Sciences, 1985
- Evidence that spectrin binds to macromolecular complexes on the inner surface of the red cell membraneJournal of Cell Science, 1980
- Isolation of phosphorylated peptides and proteins on ion exchange papersAnalytical Biochemistry, 1978
- GTP hydrolysis during methionyl-tRNAf binding to 40 S ribosomal subunits and the site of edeine inhibition.Published by Elsevier ,1978
- Monitoring of solid phase peptide synthesis by an automated spectrophotometric picrate methodAnalytical Biochemistry, 1975