Meta‐vinculin distribution in adult human tissues and cultured cells
- 20 October 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 207 (1), 139-141
- https://doi.org/10.1016/0014-5793(86)80027-8
Abstract
Meta-vinculin distribution in adult human tissue was studied by immunoblotting technique. Meta-vinculin was found in smooth (aorta wall and myometrium) and cardiac muscle, rather than in skeletal muscle, liver, kidney and cultured cells - macrophages, foreskin fibroblasts, peripheral blood lymphocytes and vascular endothelial cells. In the primary culture of smooth muscle cells from human aorta the meta-vinculin/vinculin ratio was reduced, and on the onset of cell division meta-vinculin could hardly be detected. Subcultured smooth muscle cells from human aorta did not contain meta-vinculin. The data show that the presence of meta-vinculin is characteristic of'contractile' smooth muscle cells rather than of proliferating in vitro.Keywords
This publication has 15 references indexed in Scilit:
- Cell contact- and shape-dependent regulation of vinculin synthesis in cultured fibroblastsNature, 1986
- Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cellsExperimental Cell Research, 1985
- Vinculin localization in cardiac muscleFEBS Letters, 1983
- Meta-vinculin—a vinculin-related protein with solubility properties of a membrane proteinNature, 1982
- Ultrastructure of chicken cardiac muscle as studied by double immunolabeling in electron microscopy.Proceedings of the National Academy of Sciences, 1981
- What controls smooth muscle phenotype?Atherosclerosis, 1981
- Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes.Proceedings of the National Academy of Sciences, 1980
- Microinjection and localization of a 130K protein in living fibroblasts: a relationship to actin and fibronectinCell, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- A 130K protein from chicken gizzard: Its localization at the termini of microfilament bundles in cultured chicken cellsCell, 1979