Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis
- 1 March 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 21 (3), 1031-1041
- https://doi.org/10.1128/jvi.21.3.1031-1041.1977
Abstract
The small RNAs of Moloney murine leukemia virus (M-MuLV) were fractionated into at least 15 species by 2-dimensional polyacrylamide gel electrophoresis. The pattern of small RNAs is significantly different from that of Rous sarcoma virus. A subset of the virion small RNAs is associated with the genome RNA in the 70S complex. One of the associated molecules, a cellular [mouse fibroblast 3T3, chicken embryo fibroblast and newborn rat kidney NRK cells] tRNA, is tightly bound to the genome RNA and serves as the major primer for M-MuLV RNA-directed DNA synthesis in vitro.This publication has 28 references indexed in Scilit:
- Ordered transcription of RNA tumor virus genomesJournal of Molecular Biology, 1976
- Transfer RNAs associated with the 70S RNA of AKR murine leukemia virusBiochemical and Biophysical Research Communications, 1975
- An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange celluloseAnalytical Biochemistry, 1974
- Structure and Properties of an RNA Primer for Initiation of Rous Sarcoma Virus DNA Synthesis In VitroCold Spring Harbor Symposia on Quantitative Biology, 1974
- RNA metabolism of murine leukemia virus: Detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNAJournal of Molecular Biology, 1973
- Transfer RNA Activities of Rous Sarcoma and Rous Associated VirusesNature New Biology, 1973
- The low molecular weight RNAs of Rous sarcoma virusVirology, 1970
- The low molecular weight RNAs of Rous sarcoma virusVirology, 1970
- Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis*Biochemistry, 1967
- A two-dimensional fractionation procedure for radioactive nucleotidesJournal of Molecular Biology, 1965