Dolichol(C55) mono- and pyrophosphatases of the rat liver
- 1 August 1981
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 1 (8), 619-626
- https://doi.org/10.1007/bf01116277
Abstract
Dilichol mono- and pyrophosphatase (DPase and DPPase) activity [involved in glycoprotein synthesis] was studied in rat livers. The DPase activity in microsomes had a pH optimum of 6.5, was inhibited by NaF and had a specific activity 1/5 that of the pyrophosphatase. DPPase exhibited a pH optimum of 8.0 and was inhibited by bacitracin; the hydrolysis products were dolichol monophosphatase and orthophosphate. Both phosphatases displayed high specific activities in lysosomes and plasma membranes, and were present in microsomes and outer mitochondrial membranes, but appeared to be absent from mitochondrial inner membranes and peroxisomes.This publication has 19 references indexed in Scilit:
- Enzymatic dephosphorylation of dolichyl pyrophosphate — The bacitracinsensitive, rate-limiting step for dolichyl mannosyl phosphate synthesis in rat liver microsomesBiochemical and Biophysical Research Communications, 1980
- A phosphatase acting on dolichyl phosphate in membranes from neuronal perikaryaFEBS Letters, 1980
- Possible involvement of fatty acid binding protein in peroxisomal β-oxidation of fatty acidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- A dolichyl phosphate-cleaving acid phosphatase from Tetrahymena pyriformisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- CTP-dependent dolichol phosphorylation by mammalian cell homogenatesBiochemistry, 1978
- Enzyme and phospholipid asymmetry in liver microsomal membranes.The Journal of cell biology, 1977
- Biogenesis of microsomal membrane glycoproteins in rat liver. I. Presence of glycoproteins in microsomes and cytosol.The Journal of cell biology, 1975
- Mechanism of Action of Bacitracin: Complexation with Metal Ion and C 55 -Isoprenyl PyrophosphateProceedings of the National Academy of Sciences, 1971
- Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic mediaAnalytical Biochemistry, 1970
- Studies on the Biosynthesis of CholesterolJournal of Biological Chemistry, 1962