RANTES release by human adipose tissue in vivo and evidence for depot-specific differences
Open Access
- 1 June 2009
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Endocrinology and Metabolism
- Vol. 296 (6), E1262-E1268
- https://doi.org/10.1152/ajpendo.90511.2008
Abstract
Obesity is associated with elevated inflammatory signals from various adipose tissue depots. This study aimed to evaluate release of regulated on activation, normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and omental adipose tissue from morbidly obese bariatric surgery patients and in thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES and IL-6 release in adipose tissue of lean and obese subjects and release of MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was lower than circulating levels. Ex vivo release of RANTES was greater from the gastric fat pad compared with omental (P = 0.01) and subcutaneous (P = 0.001) tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous adipose tissue in lean (P = 0.04) but not obese subjects. Indexes of obesity correlated with epicardial RANTES but not with systemic RANTES or its release from other depots. In conclusion, RANTES is released by human subcutaneous adipose tissue in vivo and in varying amounts by other depots ex vivo. While it appears unlikely that the adipose organ contributes significantly to circulating levels, local implications of this chemokine deserve further investigation.Keywords
This publication has 28 references indexed in Scilit:
- Association of serum RANTES concentrations with established cardiovascular risk markers in middle-aged subjectsInternational Journal of Cardiology, 2009
- Adipokines oversecreted by omental adipose tissue in human obesityAmerican Journal of Physiology-Endocrinology and Metabolism, 2007
- Adipose Tissue, Inflammation, and Cardiovascular DiseaseCirculation Research, 2005
- Antagonism of RANTES Receptors Reduces Atherosclerotic Plaque Formation in MiceCirculation Research, 2004
- Human Epicardial Adipose Tissue Is a Source of Inflammatory MediatorsCirculation, 2003
- Laparoscopic Adjustable Gastric Banding: Surgical TechniqueJournal of Laparoendoscopic & Advanced Surgical Techniques, 2003
- Negative role of cAMP‐dependent protein kinase A in RANTES‐mediated transcription of proinflammatory mediators through RafThe FASEB Journal, 2003
- RANTES-mediated Chemokine Transcription in Astrocytes Involves Activation and Translocation of p90 Ribosomal S6 Protein Kinase (RSK)Journal of Biological Chemistry, 2002
- Subcutaneous Adipose Tissue Releases Interleukin-6, But Not Tumor Necrosis Factor- , in VivoJournal of Clinical Endocrinology & Metabolism, 1997
- Relationship of Anthropometric Measurements of Body Fat Distribution to Metabolic Profile in Premenopausal WomenActa Medica Scandinavica, 1987