Influence of Urea on the Growth of T-Strain Mycoplasmas
- 1 May 1967
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 93 (5), 1509-+
- https://doi.org/10.1128/jb.93.5.1509-1512.1967
Abstract
T-strain mycoplasmas require urea for propagation, but urea metabolism also occurs in nonpropagating viable cultures. Ammonia results from this metabolism and alkalinizes the medium. Ammonium ions and an alkaline pH both inhibit the multiplication of T strains and reduce the viability of T strains in broth. These toxic effects of urea metabolism currently limit the growth of T strains in broth. Stock T-strain cultures are optimally maintained in continuous culture if the routine medium at pH 6.0 is supplemented with 0.05% urea and 0.002% phenol red, but an incubation temperature of 30C is preferable to 37c for subculture at 24-hr intervals.This publication has 4 references indexed in Scilit:
- CULTURE OF HUMAN GENITAL “T-STRAIN” PLEUROPNEUMONIA-LIKE ORGANISMSJournal of Bacteriology, 1962
- T-FORM COLONIES OF PLEUROPNEUMONIALIKE ORGANISMSJournal of Bacteriology, 1956