H/D Exchange and Mass Spectrometry in the Studies of Protein Conformation and Dynamics: Is There a Need for a Top-Down Approach?
- 20 August 2009
- journal article
- perspective
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 81 (19), 7892-7899
- https://doi.org/10.1021/ac901366n
Abstract
Hydrogen/deuterium exchange (HDX) combined with mass spectrometry (MS) detection has matured in recent years to become a powerful tool in structural biology and biophysics. Several limitations of this technique can and will be addressed by tapping into the ever expanding arsenal of methods to manipulate ions in the gas phase offered by mass spectrometry.Keywords
This publication has 48 references indexed in Scilit:
- Protein conformations can be probed in top-down HDX MS experiments utilizing electron transfer dissociation of protein ions without hydrogen scramblingJournal of the American Society for Mass Spectrometry, 2009
- Fast reversed-phase liquid chromatography to reduce back exchange and increase throughput in H/D exchange monitored by FT-ICR mass spectrometryJournal of the American Society for Mass Spectrometry, 2009
- Monitoring the positioning of short polycationic peptides in model lipid bilayers by combining hydrogen/deuterium exchange and electrospray ionization mass spectrometryBiochimica et Biophysica Acta (BBA) - Biomembranes, 2008
- Detection and Characterization of Altered Conformations of Protein Pharmaceuticals Using Complementary Mass Spectrometry-Based ApproachesAnalytical Chemistry, 2008
- Sequence-Specific Conformational Flexibility of SNARE Transmembrane Helices Probed by Hydrogen/Deuterium ExchangeBiophysical Journal, 2008
- Interaction of Group IA Phospholipase A2 with Metal Ions and Phospholipid Vesicles Probed with Deuterium Exchange Mass SpectrometryBiochemistry, 2008
- Intramolecular Migration of Amide Hydrogens in Protonated Peptides upon Collisional ActivationJournal of the American Chemical Society, 2005
- Thermodynamic Analysis of Subunit Interactions in Escherichia coli Molybdopterin SynthaseBiochemistry, 2005
- Methods to study protein dynamics and folding by mass spectrometryMethods, 2004
- Hydrogen/deuterium exchange electrospray ionization mass spectrometry: a method for probing protein conformational changes in solutionJournal of the American Chemical Society, 1993