5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.
- 1 February 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (2), 742-745
- https://doi.org/10.1073/pnas.72.2.742
Abstract
Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.Keywords
This publication has 13 references indexed in Scilit:
- Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.Proceedings of the National Academy of Sciences, 1975
- In vitro synthesis of methylated messenger RNA by the virion-associated RNA polymerase of vesicular stomatitis virusCell, 1974
- Sequences containing methylated nucleotides at the 5′ termini of messenger RNAs: Possible implications for processingCell, 1974
- Primary Sequence of U-1 Nuclear Ribonucleic Acid of Novikoff Hepatoma Ascites CellsJournal of Biological Chemistry, 1974
- The 5′-Terminal Nucleotide Sequences of the Double-Stranded RNA of Human ReovirusProceedings of the National Academy of Sciences, 1974
- Identification of Methylated Nucleosides in Messenger RNA from Novikoff Hepatoma CellsProceedings of the National Academy of Sciences, 1974
- Methylated Messenger RNA Synthesis In Vitro by Purified ReovirusProceedings of the National Academy of Sciences, 1974
- Methylation of Newly Synthesized Viral Messenger RNA by an Enzyme in Vaccinia VirusProceedings of the National Academy of Sciences, 1974
- Guanosine-5′-diphosphate at the 5′ termini of reovirus RNA: Evidence for a segmented genome within the virionJournal of Molecular Biology, 1971
- Studies of Nucleotide Sequences in Tobacco Mosaic Virus Ribonucleic Acid. IV. Use of Aniline in Stepwise Degradation*Biochemistry, 1966