Cloning and nucleotide sequence of cDNA for rhodopsin of the squid Todarodes pacificus
Open Access
- 8 February 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 317 (1-2), 5-11
- https://doi.org/10.1016/0014-5793(93)81480-n
Abstract
A cDNA for rhodopsin was isolated from a library constructed from poly(A)+RNA of the squid (Todarodes pacificus) retina. One positive clone with the longest insert of cDNA (3.1 kb) was selected by employing a PCR‐amplified cDNA fragment as a probe. The nucleotide sequence of the cDNA revealed a single open reading frame of 1,344 bp encoding a polypeptide (M r, 49,833), which covered a complete sequence for the squid opsin. This clone had a very long 3'‐non‐coding region (1.7 kb) including multiple polyadenylation signals, AATAAA, resembling the clones for Todarodes retinochrome and retinal‐binding protein (RALBP). The analysis of hydropathicity demonstrated the presence of seven transmembrane spanning domains, and a possible retinal‐binding site, Lys‐305, was found in the 7th domain. Todarodes rhodopsin contained characteristic sequences of PPQGY repeated in the C‐terminal region, as reported in Loligo and octopus rhodopsins. Structural comparison of those cephalopod rhodopsins is also discussed.Keywords
This publication has 20 references indexed in Scilit:
- Cloning and nucleotide sequence of cDNA for retinochrome, retinal photoisomerase from the squid retinaFEBS Letters, 1990
- Amino acid sequence of the retinal binding site of squid visual pigmentBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
- Octopus rhodopsin Amino acid sequence deduced from cDNAFEBS Letters, 1988
- Two adjacent cysteine residues in the C‐terminal cytoplasmic fragment of bovine rhodopsin are palmitylatedFEBS Letters, 1988
- Isolation and structure of a rhodopsin gene from D. melanogasterCell, 1985
- The structure of rhodopsin and the rod outer segment disk membraneTrends in Biochemical Sciences, 1983
- The structure of bovine rhodopsinEuropean Biophysics Journal, 1983
- Rhodopsin and bacteriorhodopsin: structure—function relationshipsFEBS Letters, 1982
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Three-dimensional model of purple membrane obtained by electron microscopyNature, 1975