Evidence that v-src and v-fps gene products use a protein kinase C-mediated pathway to induce expression of a transformation-related gene.
- 1 September 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (18), 7017-7021
- https://doi.org/10.1073/pnas.86.18.7017
Abstract
Induction of the transformation-related gene 9E3 by the v-src and v-fps gene products (v-Src and v-Fps) is blocked in chicken embryo fibroblasts depleted of protein kinase C (PKC). PKC agonists induce 9E3 gene expression. Protein kinase inhibitors block v-Src and v-Fps-induced 9E3 gene expression with the same dose-response curves seen for PKC agonist-induced 9E3 gene expression. These data suggest that v-Src and v-Fps use a PKC-mediated signal-transduction pathway to induce expression of the transformation-related 9E3 gene. Consistent with this hypothesis, we find that both v-Src and v-Fps rapidly induce phosphorylation of a 67-kDa PKC substrate.This publication has 36 references indexed in Scilit:
- Dissociation of inositol trisphosphate from diacylglycerol production in Rous sarcoma virus-transformed fibroblasts.The Journal of cell biology, 1989
- Stimulus-dependent myristoylation of a major substrate for protein kinase CNature, 1988
- Transformation by Rous sarcoma virus induces a novel gene with homology to a mitogenic platelet proteinCell, 1987
- Differential effects of protein kinase C activators on carbamylcholine- and high K+-induced rises in intracellular free calcium concentration in cultured adrenal chromaffin cellsBiochemical and Biophysical Research Communications, 1986
- The phorbol ester receptor: a phospholipid-regulated protein kinaseBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1985
- Activation of the transformation potential of the cellular fps geneCell, 1985
- The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF 1Cell, 1985
- 1-(5-isoquinol inesulfonyl)-2-methylpiperazine (H-7) is a selective inhibitor of protein kinase c in rabbit plateletsBiochemical and Biophysical Research Communications, 1984
- Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cellsBiochemical and Biophysical Research Communications, 1984
- Simian Sarcoma Virus onc Gene, v- sis , Is Derived from the Gene (or Genes) Encoding a Platelet-Derived Growth FactorScience, 1983