Calcofluor White Alters the Assembly of Chitin Fibrils in Saccharomyces cerevisiae and Candida albicans Cells

Abstract

In the presence of calcofluor white, budding scars and dividing cross-walls of Saccharomyces cerevisiae exhibited fluorescence, indicating that the brightener was a specific marker of fungal chitin. In addition, incubation of cells in the presence of the brightener did not stop protein and wall-polymer formation, but abnormal deposition of chitin occurred. Chitin synthesis was normal in regenerating protoplasts of Candida albicans in the presence of calcofluor, but formation of the crystalline lattice was blocked. These results suggest that crystallization of nascent subunits may occur by a self-assembly mechanism that was blocked by the stain.