Phospholipid Metabolism in Ferrobacillus ferrooxidans
- 1 July 1969
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 99 (1), 142-150
- https://doi.org/10.1128/jb.99.1.142-150.1969
Abstract
The lipid composition of the chemoautotroph Ferrobacillus ferrooxidans has been examined. Fatty acids represent 2% of the dry weight of the cells and 86% of the total are extractable with organic solvents. About 25% of the total fatty acids are associated with diacyl phospholipids. Polar carotenoids, the benzoquinone coenzyme Q-8, and most of the fatty acids are present in the neutral lipids. The phospholipids have been identified as phosphatidyl monomethylethanolamine (42%), phosphatidyl glycerol (23%), phosphatidyl ethanolamine (20%), cardiolipin (13%), phosphatidyl choline (1.5%), and phosphatidyl dimethylethanolamine (1%) by chromatography of the diacyl lipids, by chromatography in four systems of the glycerol phosphate esters derived from the lipids by mild alkaline methanolysis, and by chromatographic identification of the products of acid hydrolysis of the esters. No trace of phosphatidylserine (PS), glycerolphosphorylserine, or serine could be detected in the lipid extract or in derivatives of that extract. This casts some doubt on the postulated involvement of PS in iron metabolism. After growth in the presence of 14C and 32P, there was essentially no difference in the turnover of either isotope in the glycerolphosphate ester derived from each lipid in cells grown at pH 1.5 or 3.5.Keywords
This publication has 22 references indexed in Scilit:
- Phospholipid metabolism during bacterial growthJournal of Lipid Research, 1969
- Phospholipids from the chemoautotroph Ferrobacillus ferrooxidansBiochemical and Biophysical Research Communications, 1967
- FERROUS IRON OXIDATION BY FERROBACILLUS FERROOXIDANS: PURIFICATION AND PROPERTIES OF Fe++-CYTOCHROMEcREDUCTASECanadian Journal of Biochemistry, 1967
- ISOLATION AND CHARACTERIZATION OF BACTERIAL MEMBRANES*,†Transactions of the New York Academy of Sciences, 1967
- Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipidsJournal of Lipid Research, 1966
- Thin-layer chromatography of long-chain tertiary amines and related compoundsJournal of Chromatography A, 1965
- A simple, specific spray for the detection of phospholipids on thin-layer chromatogramsJournal of Lipid Research, 1964
- ELECTRON TRANSPORT SYSTEMS OF CHEMOAUTOTROPH FERROBACILLUS FERROOXIDANS .1. CYTOCHROME C-CONTAINING IRON OXIDASE1963
- The biosynthesis of choline and its relation to phospholipid metabolismBiochimica et Biophysica Acta, 1960
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959