RECOVERY CHANGES IN TRANSPLANTED ANTERIOR PITUITARY CELLS STRATIFIED IN THE ULTRA-CENTRIFUGE

Abstract

Anterior pituitary tissue which has had its cell contents stratified through 20 to 60 minutes of rotation in an ultra-centrifuge with a displacement pull of about 400,000 times that of gravity, returns in many instances to normal appearance, displaying the characteristic basophile and oxyphile cells, when transplanted into young rats. An hour seems to be nearly the maximum time such tissues can be thus rotated and remain viable. Nuclei forced completely to one side of the cell rapidly resume their normal location near the cell center, with their displaced chromatin contents apparently restored to normal distribution. The Golgi apparatus, concentrated by the rotation into liquid drop at the centripetal side of the cell, is the last part of the cell complex to resume its characteristic appearance, which is that of a network. A mucoid cyst with hypertrophied and extremely active epithelial wall cells, most of them ciliated, is described and compared with the type of cyst that is occasionally found in the normal pituitary.