Identification of Tn4430, a transposon of Bacillus thuringiensis functional in Escherichia coli
- 1 July 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 204 (1), 52-57
- https://doi.org/10.1007/bf00330186
Abstract
The mobile genetic element Tn4430, originating from the gram-positive bacterium, Bacillus thuringiensis, and previously described as the Th-sequence, is the first transposon isolated from the genus Bacillus. In the present work a gene (APH-III) conferring resistance to kanamycin was inserted into this 4.2 kb transposon. Transposition experiments showed that Tn4430ΩAPH-III could transpose in the gram-negative host Escherichia coli when its insertion functions were supplied by an intact copy of Tn4430. By transposing Tn4430ΩAPH-III directly onto pBR322, it was possible to determine the nucleotide sequence of the terminal inverted repeats of Tn4430 and of the target DNA site. Identical 38 bp in inverted orientation are situated at each end of the transposon and there is a direct duplication of 5 bp at the insertion site. Thus, it is clear that Tn4430 is closely related to the transposons belonging to the Tn3 family (class II elements).This publication has 63 references indexed in Scilit:
- Recent aspects of genetic manipulation in Bacillus thuringiensisBiochimie, 1985
- A physical and functional analysis of Tn917, a Streptococcus transposon in the Tn3 family that functions in BacillusPlasmid, 1984
- Cointegrate formation mediated by Tn9Journal of Molecular Biology, 1983
- Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5''-aminoglycoside phosphotransferase type IIIGene, 1983
- TRANSPOSABLE ELEMENTS IN PROKARYOTESAnnual Review of Genetics, 1981
- Isolation of plasmid deletion mutants and study of their instabilityPlasmid, 1981
- Identification of a Sex-factor-affinity Site in E. coli asCold Spring Harbor Symposia on Quantitative Biology, 1981
- Terminal nucleotide sequences of Tn551, a transposon specifying erythromycin resistance in Staphylococcus aureus: Homology with Tn3Plasmid, 1980
- Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322Published by Cold Spring Harbor Laboratory ,1979
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969