Preparative-scale reductions of cyclic ketones and aldehyde substrates of horse liver alcohol dehydrogenase with in situ sodium dithionite recycling of catalytic amounts of NAD

Abstract

During preparative-scale reductions of cyclic ketone and aldehyde substrates of horse liver alcohol dehydrogenase, up to 105-fold recycling of catalytic amounts of coenzyme has been achieved by in situ regeneration of NADH with sodium dithionite; the results indicate that >70% yields of the corresponding alcohols will be obtained provided the rate of reduction of a carbonyl compound is not less than 0·01 times that of cyclohexanone.