ADENO-ASSOCIATED SATELLITE VIRUS INTERFERENCE WITH THE REPLICATION OF ITS HELPER ADENOVIRUS

Abstract

Satellite virus interferes with the replication of its helper adenovirus. No interferon-like soluble substance could be detected in satellite-infected cultures and other DNA- and RNA-containing viruses were not inhibited by coinf ection with satellite virus under conditions which reduced adenovirus yields by more than 90% in monkey cells. The interference with adenovirus replication was virus-mediated, for it could be abolished by pretreating satellite preparations with specific antiserum, ultraviolet light or heating at 80[degree]C for 30 min. Eight hours after adenovirus infection, the replication of adenovirus was no longer sensitive to satellite interference, even though the satellite virus was enhanced as effectively as if the cells were infected simultaneously with both viruses. When cells were infected either with adenovirus alone or with adenovirus plus satellite, the same proportion of cells plated as adenovirus infectious centers. However, the number of plaque-forming units of adenovirus formed per cell in the satellite-infected cultures was reduced by approximately 90%, the same magnitude of reduction noted in whole cultures co-infected with satellite and adenovirus. This suggested that all cells infected with the 2 viruses seemed to be producing a reduced quantity of adenovirus.