Separation of Six DNA Bases by Ion Pair--Reversed Phase High Pressure Liquid Chromatography
- 1 September 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 17 (9), 531-534
- https://doi.org/10.1093/chromsci/17.9.531
Abstract
An ion pair-reversed phase chromatography system is described which separates six of the bases normally found in prokaryotic and/or eukaryotic ONA from each other and from uracil, the characteristic base found in RNA. The effect of varying phi and the percentage of methanol in the column buffer is described. The addition of the ion-pairing reagent heptane sulfonate was shown to be necessary to achieve separation of all of these bases.This publication has 8 references indexed in Scilit:
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